| 質粒名稱: | pLB2 CAG P2Gm |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 19752 |
| 存儲實驗室: | Richard Hynes |
|
載體骨架基本信息 |
|
| 載體名稱: | LB2 |
| 載體出品公司: | -- |
| 載體抗性: | 氨芐青霉素 |
| 空載體大小: | 10552 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳動物表達，小鼠靶向，慢病毒，RNA干擾 |
| 篩選標記: | 嘌呤霉素 |
| 高拷貝/低拷貝: | 低拷貝 |
| 生長條件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | NotI(未破壞) |
| 3' 克隆位點: | PmeI(未破壞) |
| 5' 測序引物 | GFP miR primer (GCTGGAGTTCGTGACCGCC) |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pLB2 CAG P2Gm was a gift from Richard Hynes (Addgene plasmid # 19752 ; //n2t.net/addgene:19752 ; RRID:Addgene_19752) |
| 參考文獻部分: | A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577 |
買家導航
pLB2 CAG P2Gm載體質粒基本信息
pLB2 CAG P2Gm質粒圖譜載體圖譜和pLB2 CAG P2Gm載體序列質粒序列多克隆位點信息
pLB2 CAG P2Gm質粒載體簡介
pLB2 CAG P2Gm質粒序列載體序列
LOCUS pLB2 CAG P2Gm 10656 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLB2 CAG P2Gm
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10656)
AUTHORS Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
TITLE A system for Cre-regulated RNA interference in vivo.
JOURNAL Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
PUBMED 18779577
REFERENCE 2 (bases 1 to 10656)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..10656
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 355..372
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 489..510
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 525..555
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 563..579
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 568..590
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 587..603
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 587..603
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
LTR 869..1049
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1096..1221
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1720..1953
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2480..2597
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 3119..3422
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3424..3701
/label=chicken beta-actin promoter
intron 3703..4720
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 4644..4666
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 4728..4747
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 4809..4818
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4815..5411
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 5311..5331
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 5433..5498
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
regulatory 5508..5517
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5514..6233
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5559..5580)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5820..5839)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6167..6188
/label=EGFP-C
/note="EGFP, forward primer"
ncRNA 6283..6404
/label=5' miR-30a
/note="sequence upstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 6431..6445
/label=miR-30a loop
/note="loop from the the 71-nt precursor of the human
miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 6473..6599
/label=3' miR-30a
/note="sequence downstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
misc_feature 6685..7273
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6738..6758)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7156..7167)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7276..7292)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7277..7293)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 8436..8643
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
LTR 8662..8842
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8904..9492)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8984..9003)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9663..10523)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10286..10305
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10524..10628)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagtcat gttctttcct gcgttatccc ctgattctgt ggataaccgt
301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
601 gaccatgatt acgccaagcg cgcaattaac cctagcttaa tgtagtctta tgcaatactc
661 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
721 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
781 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
841 tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga tctgagcctg
901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
1021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
1081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
1141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
1201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
1261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
1321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
1381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
1441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
1501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
1561 gccggccgcg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1621 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1681 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1741 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1801 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1861 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1921 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1981 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
2041 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
2101 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
2161 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
2221 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
2281 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
2341 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
2401 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
2461 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
2521 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
2581 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac
2641 cgggcccggt gctttgctct gagccagccc accagtttgg aatgactcct ttttatgact
2701 tgaattttca agtataaagt ctagtgctaa atttaatttg aacaactgta tagtttttgc
2761 tggttggggg aaggaaaaaa aatggtggca gtgttttttt cagaattaga agtgaaatga
2821 aaacttgttg tgtgtgagga tttctaatga catgtggtgg ttgcatactg agtgaagccg
2881 gtgagcattc tgccatgtca ccccctcgtg ctcagtaatg tactttacag aaatcctaaa
2941 ctcaaaagat tgatataaac catgcttctt gtgtatatcc ggtctcttct ctgggtagtc
3001 tcactcagcc tgcatttctg ccagggcccg ctctagccgt taattaactg caggaatcta
3061 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
3121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
3181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
3241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
3301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
3361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
3421 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
3481 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
3541 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
3601 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
3661 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc tgcgacgctg
3721 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
3781 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
3841 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
3901 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
3961 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
4021 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
4081 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
4141 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
4201 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
4261 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
4321 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
4381 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
4441 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
4501 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
4561 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
4621 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
4681 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
4741 tattgtgctg tctcatcatt ttggcaaaga attgatttga taccgcgggc ccgggatctc
4801 gaaccggtgc caccatggtg gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
4861 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
4921 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
4981 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
5041 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
5101 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
5161 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
5221 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
5281 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
5341 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
5401 agcccggtgc caaacagaaa attgtggcac cagtgaaaca gactttgaat tttgaccttc
5461 tcaagttggc gggagacgtc gagtccaacc ctgggcccgg cccggtcgcc accatggtga
5521 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
5581 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
5641 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
5701 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
5761 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
5821 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
5881 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
5941 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
6001 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
6061 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
6121 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
6181 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taggcggccg
6241 caagccttgt taagtgctcg cttcggcagc acatatacta tgttgaatga ggcttcagta
6301 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
6361 aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgagc tcccgtgaat
6421 tggaatccta gtgaagccac agatgtagga ttccaattca gcgggagcct gcctactgcc
6481 tcggaattca aggggctact ttaggagcaa ttatcttgtt tactaaaact gaataccttg
6541 ctatctcttt gatacatttt tacaaagctg aattaaaatg gtataaatta aatcactttt
6601 ttcaattgga agactaatgc gtttaaacca catgtttaag ggttccggtt ccactaggta
6661 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
6721 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
6781 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
6841 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
6901 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
6961 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
7021 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
7081 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
7141 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
7201 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
7261 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
7321 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
7381 ggaggaggtg ggttttccag tcacacctca ggtaccaagc atggggtaaa gtactgttct
7441 catcacatca tatcaaggtt atataccatc aatattgcca cagatgttac ttagcctttt
7501 aatatttctc taatttagtg tatatgcaat gatagttctc tgatttctga gattgagttt
7561 ctcatgtgta atgattattt agagtttctc tttcatctgt tcaaattttt gtctagtttt
7621 attttttact gatttgtaag acttcttttt ataatctgca tattacaatt ctctttactg
7681 gggtgttgca aatattttct gtcattctat ggcctgactt ttcttaatgg ttttttaatt
7741 ttaaaaataa gtcttaatat tcatgcaatc taattaacaa tcttttcttt gtggttagga
7801 ctttgagtca taagaaattt ttctctacac tgaagtcatg atggcatgct tctatattat
7861 tttctaaaag atttaaagtt ttgccttctc catttagact tataattcac tggaattttt
7921 ttgtgtgtat ggtatgacat atgggttccc ttttattttt tacatataaa tatatttccc
7981 tgtttttcta aaaaagaaaa agatcatcat tttcccattg taaaatgcca tatttttttc
8041 ataggtcact tacatatatc aatgggtctg tttctgagct ctactctatt ttatcagcct
8101 cactgtctat ccccacacat ctcatgcttt gctctaaatc ttgatattta gtggaacatt
8161 ctttcccatt ttgttctaca agaatatttt tgttattgtc ttttgggctt ctatatacat
8221 tttagaatga ggttggcaag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
8281 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc cagctgcttt
8341 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
8401 tagggaaccc actgcttaag cctcataaag ctcgactgtg ccttctagtt gccagccatc
8461 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
8521 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
8581 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
8641 ggatgcggtc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
8701 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
8761 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
8821 tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
8881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
8941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
9001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
9061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
9121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
9181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
9241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
9301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
9361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
9421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
9481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
9541 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
9601 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
9661 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
9721 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
9781 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
9841 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
9901 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
9961 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
10021 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
10081 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
10141 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
10201 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
10261 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
10321 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
10381 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
10441 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
10501 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
10561 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
10621 gttccgcgca catttccccg aaaagtgcca cctgac
//